Plasmid

Part:BBa_K5319673:Design

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-09-30)


lacUV5-2nt-RS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 425
    Illegal XbaI site found at 843
    Illegal PstI site found at 1207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1207
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 425
    Illegal XbaI site found at 843
    Illegal PstI site found at 1207
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 425
    Illegal XbaI site found at 843
    Illegal PstI site found at 1207
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2544
    Illegal SapI site found at 1461


Design Notes

The target site of mutation is chosen referring to the domain function analysis in the article "Hydrolytic endonucleolytic ribozyme (HYER)is programmable for sequence-specific DNA cleavage", which explains the function of the 2nt-bulge as the structure which DNA substrate stabilizer to help with locating. The 14-nt recruiting sequence is modified from the sequence in "Hydrolytic endonucleolytic ribozyme (HYER)is programmable for sequence-specific DNA cleavage" corresponding to the site we want it to recognize.


Source

HYER(hydrolytic endonucleolytic ribozymes), identified from group II-C introns in public bacterial genome database in the article "Hydrolytic endonucleolytic ribozyme (HYER)is programmable for sequence-specific DNA cleavage".

References